Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| target genes | prime forward/reverse | primer sequence (5′ → 3′) |
|---|---|---|
| GAPDH | forward | TGCACCACCAACTGCTTAGC |
| reverse | GGCATGGACTGTGGTCATGAG | |
| ZO-1 | forward | GCTCCTGCTATCCACCTA |
| reverse | CCTGAATCGGGCTCTCATAC | |
| occludin | forward | GCACTTGTTAAGGCAGCAG |
| reverse | ACGGTAAGCATTGGCGCA | |
| claudin-1 | forward | GTGAACCGTGGACGGAAA |
| reverse | CTCCGCTGATTCACAGATTTC | |
| caspase-3 | forward | TGGAATTGATGCGTGATGTT |
| reverse | GGCAGGCCTGAATAATGAAA |
