Skip to main content
. 2020 Aug 18;11(4):e01902-20. doi: 10.1128/mBio.01902-20

FIG 4.

FIG 4

Pgp3 is required for establishing persistent ocular infection in nonhuman primates. (A) Schematic depiction of the predicted ORF of Pgp3 in C. trachomatis ocular strain A2497. The pgp3 mutant contains an inactivating stop codon at C304T. (B) Western blotting showed that Pgp3 was not expressed in the A2497 pgp3– strain. (C) A2497 and A2497 pgp3– strains exhibit similar growth kinetics in McCoy cells. One-step growth curves of McCoy cells were determined. McCoy cells were infected at an MOI of 0.5. Cells were harvested at different times postinfection, and the number of rIFU was determined. Mean and standard deviation are shown (n = 3). (D) Ocular infection of macaques (n = 4) infected with the A2497 and A2497 pgp3– strains. (E) Ocular clinical disease scores of macaques (n = 4) infected with the A2497 and A2497 pgp3– strains. (F) Representative images of macaque eyes infected with A2497 and A2497 pgp3– strains.