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. 2020 Aug 11;11(4):e00789-20. doi: 10.1128/mBio.00789-20

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Copyright © 2020 Matamoros et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 10 — Nonredundant roles of proteins pE199L and pE248R in virus entry. (A) Equal amounts of control vE199Li⁺ and defective vE199Li⁻ virions (top) or vE248Ri⁺ and vE248Ri⁻ particles (bottom) were analyzed by immunoblotting with antibodies to pE199L and pE248R proteins. (B) Membrane fractions from Vero cells infected with recombinant vE248Ri in the presence (+IPTG) or absence (−IPTG) of inducer were subjected to detergent solubilization and analyzed by sucrose density gradient (5% to 20%) centrifugation. The fractions of both gradients were analyzed by electrophoresis and immunoblotting with antibodies to pE199L and pE248R proteins. As a reference, the sedimentation profiles of the molecular weight markers carbonic anhydrase (CA, 29 kDa), bovine serum albumin (BSA, 66 kDa), alcohol dehydrogenase (AD, 150 kDa), and β-amylase (AM, 200 kDa) are shown.