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. 2020 Aug 11;11(4):e01504-20. doi: 10.1128/mBio.01504-20

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Copyright © 2020 De Palma et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — Major glycolytic enzymes are differentially ubiquitylated in the absence of Gcn5p and Ubp8p. (A) Glycolytic pathway in S. cerevisiae. Enzymes are indicated according to the ubiquitylation color code in Fig. 2B. The color code palette found in ubp8Δ, gcn5Δ, and ubp8Δ gcn5Δ strains is compared to WT for the indicated enzymes. (C) RT-qPCR of PFK1, PFK2, and PYC1 mRNA expression respect to actin in WT, ubp8Δ, and gcn5Δ strains. (D) Ubiquitylation pattern of Pfk1p the essential enzyme required for initiation of glycolytic flux. His6Ub-Pfk1pmyc version (∼120 kDa) was analyzed by Western blotting in the indicated strains hybridized with anti-myc and anti-tubulin (55 kDa) as internal loading control. The lower panel shows the eluted profiles of His6Ub-Pfk1pmyc in different strains.