FIG 4.

Suppression of p62 inhibits HPV genome amplification and increases GATA4 expression in CIN612 cells. (a) Western blot analysis of p62 and GAPDH levels in CIN612 cells transiently transduced with five different shRNA lentiviruses against p62. (b) Southern blot analysis for HPV31 episomes in CIN612 cells with stable p62 knockdown following differentiation in high-calcium media for the indicated times (in hours). (c) Western blot analysis of p62, GATA4, and GAPDH protein levels in mock, scrambled control, and two stably selected p62 knockdown CIN612 cells. (d) Western blot analysis of p62, GATA4, and GAPDH protein levels in the cells described in the legend for panel c following differentiation in high-calcium media for the indicated times (in hours). In the lane of mock cells at 0 h of differentiation, there was likely a bubble over the GATA4 band that occurred during the transfer process. The presence of GATA4 in undifferentiated cells is also shown in Fig. 5c. All results are representative of observations from two or three independent experiments.