FIG 4.

Dynamics of pre-epidemic ZIKV in the midguts of A. aegypti and C. quinquefasciatus. ZIKV-Cambodia titers in the midguts of A. aegypti Rock strain (green) and C. quinquefasciatus JHB strain (blue) mosquitoes by qRT-PCR in log₁₀ (mean p-e/midgut) at 12-hour intervals post-exposure. Average titers per mosquito from pools of 25 mosquitoes per time point per replicate are presented as ● symbols, and boxplots indicate median titers and interquartile ranges. Samples falling within the gray band are of indeterminate infectious status; samples above the band are considered positive, and samples below the band are considered negative. ZIKV titer limits for sample categories are adjusted to reflect pooled samples. Results are from N = 3 independent biological replicates. ZIKV titers in the midgut were analyzed by ANOVA using the following model: Y_ijklm = μ + strains_j + hpe_k + replicate_l + species:hpe_m. In the midgut analysis, there was a significant interaction between strain and hpe (P = 0.006) and a significant effect of replicate (P = 0.012). The assumption of normality was violated. Uppercase letters indicate significant difference between groups (P < 0.05) as determined by Tukey’s HSD post hoc analysis.