TABLE 2.

Sequences of primers and oligonucleotides

Identification	Sequence (5′→3′)
Sanger sequencing primer
U6 forward primer	CGTGACGTAGAAAGTAATAATTTCTTGGG
sgRNA sequences targeting gene:
POR	ACATGCCTCGCATCCCGTAG
RETSAT	TCAGCAGCCCACACCTGTCG
KEAP1	GCAATGAACACCATCCGAAG
SLC52A1	TGGTCACGGCACTGGTGAA
SLC52A2	AGCACCCACGCCCGCCCGTC
SLC52A3	CGGACGAGAAGAGCCGCAGC
Amplification primers for Sanger sequencing
SLC52A1 forward primer	AGAAAGACCAACCAGCCCTG
SLC52A1 reverse primer	GCCTCCCCTCATACCTCTCT
SLC52A2 forward primer	GGCTCCACTCGCTCCTTC
SLC52A2 reverse primer	AATGCAACTGTCCTGCCACT
SLC52A3 forward primer	TCCCAGCTGTTTCCCTTCTA
SLC52A3 reverse primer	GGTGGGGAAGTCCTCATTG