Fig. 5. RACK7 directly regulates CIITA and genes involved in vesicular transportation through its chromatin binding.

(A) Flow cytometric analysis of HLA-DR antibody–stained R6, R6^{WT H3.3}, R6^{RACK7 KO}, R6^{CIITA KO}, R6^{WT H3.3 + CIITA KO}, and R6^{RACK7 KO + CIITA KO} cells. All cells contain YFP transgene (detailed in Materials and Methods). YFP-positive cells were used to analyze the cell surface expression of HLA-DR. (B) RT-qPCR of gene expressions in R6, R6^{WT H3.3}, and R6^{RACK7 KO} cells. Data are represented as means ± SD from three biological replicates, ***P < 0.001, two-tailed Student’s t test. (C) Flow cytometric analysis of HLA-DR antibody–stained R6^{QKI KO}, R6^{WT H3.3 + QKI KO}, and R6^{RACK7 KO + QKI KO} cells, compared with their parental cells in (A). All cells contain YFP transgene (detailed in Materials and Methods). YFP-positive cells were used to analyze the cell surface expression of HLA-DR. (D) Flow cytometric analysis of the HLA-DR antibody–stained R6 (top), R6^{WT H3.3} (middle), and R6^{RACK7 KO} (bottom) lenti-CRISPR KO GFAP, VIM, and OCIAD2, respectively. All cells contain YFP transgene (detailed in Materials and Methods). YFP-positive cells were used to analyze the cell surface expression of HLA-DR.