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. 2020 Jul 24;6(30):eabb4429. doi: 10.1126/sciadv.abb4429

Fig. 4. In vivo delivery of Cre recombinase to the Ai14 mouse brain to induce gene recombination.

Fig. 4

(A) Schematic illustration of mixed LNP formulation using NT1-O14B and PBA-Q76-O16B for GFP-Cre protein delivery into the brain. (B) Fluorescence image of the brain slices of Ai14 mice treated with (−27)GFP-Cre in different LNP formulations. Ai14 mouse was intravenously injected with (−27)GFP-Cre complexed with NT1-O14B:PBA-Q76-O16B = 3:7, 10:0, or 0:10 LNPs. After 3 weeks, the group of NT1-O14B:PBA-Q76-O16B = 3:7 showed tdTomato expression indicative of Cre-mediated recombination in cerebral cortex, hippocampus, and cerebellum. Red, tdTomato. The white arrows in the hippocampus 3:7 subpanel indicate tdTomato+ cells. Blue, DAPI. Scale bars, 100 μm.