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. 2020 Aug 20;13:426. doi: 10.1186/s13071-020-04279-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Immunolocalization of inducible nitric oxide synthase (iNOS) a 3-nitrotyrosine (3-NT) in pinnae of mice infected with Trichobilharzia regenti. The iNOS signal (red) surrounded the penetrating schistosomula 8 h post-infection (hpi) and spread also in the adjacent epidermal area (white arrow). Leukocytes infiltrated the lesion 24 hpi, but neither iNOS nor 3-NT signals were detected at this or later (48 hpi) time points. The space occupied by schistosomula is marked with white line in DAPI images. Three mice per time point and at least two schistosomula-positive slides per mouse were analyzed. The faint red signal observed 24 and 48 hpi represents a non-specific binding of the rabbit polyclonal antibody to the schistosomula and was observed also in control samples incubated with negative rabbit serum (not shown). Scale-bars: 50 μm