Fig. 5.

Viability of Trichobilharzia regenti schistosomula after 48-h treatment by donors of nitric oxide or peroxynitrite. Nitric oxide was released from NOR-5 (0.1 and 0.5 mM) and peroxynitrite from SIN-1 (1.5 and 3 mM). The viability was assessed by methylene blue staining (a) and analysis of lactate production (b). NOR-5 treated schistosomula (orange boxplots) remained mostly unstained, i.e. viable, but they increased production of lactate in a dose-dependent manner. However, schistosomula treated by SIN-1 (green boxplots) evinced decreased viability as well as reduced production of lactate. The graphs show pooled data from three experiments, each performed in quadruplicates. Data were evaluated by one-way ANOVA followed by Šidák’s test; *P < 0.05, ***P < 0.001