Figure 4. The mitochondrial Glrx5 gene is a direct miR-152 target.

a-b) Enrichment analysis of predicted miR152 ‘seed sequence’ motifs in the downregulated gene pool and upregulated gene pools. c) mRNA expression levels of 23 putative miR-152 targets represented as a heatmap. d) Representative Western blot analysis of miR-152 putative target Glrx-5 and densitometry analysis of the protein expression levels. Comparison by upaired student’s t-test; n=4 per group. Gapdh was used as a loading control. Numbers indicate the molecular weight marker size (kDa). e) Sequence complementarity between miR152 ‘seed sequence’ and Glrx5 3’-UTR. f) Pre-miR-152 mimic or pre-miR-control mimics were co-transfected with Luciferase reporter vector carrying the Glrx5 3′-UTR or a vector without the predicted binding site of miR-152. n=3 independent experiments. Comparison by 2-way ANOVA. All box-and-whisker plots show the minimum, the 25^th percentile, the median, the 75^th percentile, and the maximum.