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. 2020 Aug 20;12:125. doi: 10.1186/s13148-020-00914-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — PRDM8 knock out leads to impaired neuronal differentiation. a Representative phase contrast pictures of neuronal differentiations on day 13. Control cells reveal typical aggregates of immature sensory neurons, which are absent in the PRDM8 knockout cells. Scale bar, 200 μM. b Quantitative RT-PCR analysis after 10 days of neuronal differentiation shows upregulation of neuronal markers in control cells, but not in PRDM8 knockouts (color code depicts mean fold change versus GAPDH). c Gene expression changes in RNA-Seq after 27 days of peripheral neuron differentiation (RPM, reads per million; genes with a log2 fold change above 2 or below − 2 are depicted). d CpG sites that are either 20% hypermethylated (red, 1511 CpGs) or hypomethylated (blue, 1738 CpGs) in the PRDM8^−/− clone compared to the isogenic control. e Epigenetic age of control and knockout iPSCs after neuronal differentiation was close to 0 year.