Table 3.

Use case 2 for workflow segments (campaigns): Fungal isolates, barcoding and phenotypic trait description: Domains 1–3: transformation (TF), measurement (ME) and transaction design (TA) elements (E 1–n) and method information (MI) elements (E 1–3)

Transformation design (TF)	Measurement design (ME)	Transaction design (TA)	Method information (MI)
Workflow segment 1
E 1: site number/ID	E 1: GPS data	E 1: box ID	E 1 (transformation): sampling protocol
E 2: borehole number/ID		E 2: container ID	E 2 (measurement): GPS (time, space) of borehole at site protocol
E 3: soil horizon type/depth definition			E 3 (transaction): sample into container transfer protocol
E 4: replicate/aliquot number/ID
Workflow segment 2
E 1: (sub-)culture generation number	E 1: fungal colony growth rate	E 1: microplate storage rack ID	E 1: fungal strain isolation and cultivation protocol
E 2: culture medium type		E 2: microplate ID	E 2: fungal culture growth measurement protocol
E 3: culture medium type variation		E 3: microplate-internal position coordinate	E 3: 1: fungal culture translocation-inoculation measurement protocol
E 4: culture replicate number		E 4: aliquot number/ID
Workflow segment 3
E 1: DNA extraction	E 1: DNA extract concentration and purity	E 1: microplate storage rack ID	E 1: nucleic acid extraction protocol
		E 2: microplate ID	E 2: nucleic acid quantity/quality measurement protocol
		E 3: microplate-internal position coordinate	E 3: intermediate object transfer into container
		E 4: aliquot number/ID
Workflow segment 4
E 1: DNA amplification	E 1: PCR product concentration and purity	E 1: microplate storage rack ID	E 1: DNA amplification protocol
		E 2: microplate ID	E 2: DNA amplificate quantity/quality measurement protocol
		E 3: microplate-internal position coordinate	E 3: intermediate object transfer into container protocol
		E 4: aliquot number/ID
Workflow segment 5
E 1: DNA isolate storage	E 1: PCR product concentration	E 1: room number/ID	E 1: DNA isolates storage protocol
		E 2: freezing device ID	E 2: storage parameters control protocol
		E 3: object/product container ID	E 3: product transfer into container protocol
		E 4: aliquot number/ID
Workflow segment 6
E 1: DNA amplicon storage	E 1: PCR product storage temperature	E 1: room number/ID	E 1: DNA amplicon storage protocol
		E 2: freezing device ID	E 2: storage parameters control protocol
		E 3: object/product container ID	E 3: product transfer into container protocol
		E 4: aliquot number/ID
Workflow segment 7
E 1: fungal culture staining	E 1: fungal trait 1	E 1: culture storage room number	E 1: culture preparation (staining) for light microscopy protocol
	E 2: fungal trait 2	E 2: culture storage rack ID	E 2: culture measurement protocol with checklist of morphological traits (to be recorded in measurement values database)
	E 3: fungal trait 3	E 3: culture storage shelf number	E 3: product transfer onto slide for LM protocol
	E 4: fungal trait 3 + n	E 4: storage box ID
Workflow segment 8
E 1: fungal culture storing	E 1: culture storage temperature	E 1: culture storage room number	E 1: culture storage protocol
	E 2: culture storage humidity	E 2: culture storage rack ID	E 2: storage parameters control protocol
	E 3: culture storage light	E 3: culture storage shelf number	E 3: culture transfer into container protocol
		E 4: storage box ID