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. 2020 Jul 22;6(30):eabb6049. doi: 10.1126/sciadv.abb6049

Fig. 1. Screening of cholinium-based bioactive IL-RNA complex for enhanced epidermal accumulation.

Fig. 1

(A) CD spectra of siRNA in phosphate-buffered saline (PBS) following incubation with IL (50% v/v) for 30 min and dialysis for 72 hours. (B) Representative native gel image of siRNA following IL incubation. bp, base pair. (C) Representative confocal images of siRNA (red) in the different skin layers (a) stratum corneum (SC), (b) epidermis, and (c) dermis, in the presence of IL combination (CAGE + CAPA) mixed at a ratio 1:1 following 24 hours of incubation. Left to right: Merged, Cy5, differential interference contrast (DIC). Scale bars, 50 μm. (D and E) Transport of Cy5-labeled siRNA in the presence of individual ILs with concentration of 50% (v/v) (D) and combination of ILs at 50% (v/v) (E) into the different layers of skin determined by the tape-stripping method (n = 3). Data are averages ± SEM and were determined to be nonparametric by normality test and statistics by Kruskal-Wallis test for (D) and (E). *P < 0.05.