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. 2020 Aug 20;18:130. doi: 10.1186/s12964-020-00615-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — SRSF1 binds to miR-1246. a qRT-PCR detection of miR-1246 in IgG, GAPDH, SRSF1, EIF3B, and TIA1 immunoprecipitants of PANC-1 lysate (n = 3, *p < 0.001, student t-test). b-c EMSA detection of the SRSF1-miR-1246 complex (hot probe: IRD-800 labeled miR-1246 mimics; cold probe: miR-1246 mimics, n = 3). Direct binding of GST-SRSF1 and miR-1246 (b); and concentration-dependent competition between the cold and hot miR-1246 probe for binding to GST-SRSF1 (c). d Semi-quantification of SRSF1 and miR-1246 binding in C and calculated dissociation constant (n = 3)