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. 2020 Aug 20;17:244. doi: 10.1186/s12974-020-01919-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Microglial/macrophage activation and polarization in the dentate gyrus (DG) region of 2VO rats. a, b Representative images of CD68 expression in Iba-1⁺cells in hippocampal DG region of sham and 2VO rats at 1 week (a) and 8 weeks (b). c, d Representative images of CD206 expression in Iba-1⁺cells in hippocampal DG region of sham and 2VO rats at 1 week (c) and 8 weeks (d) by immunofluorescence staining. Scale: 40 μm. e Quantification of the Iba-1⁺ cell number in hippocampal DG region. f Quantification of the total polarized microglia/macrophage number in hippocampal DG region. g Quantification of the ratio of CD68 in Iba-1⁺cells in hippocampal DG region. h Quantification of the ratio of CD206 in Iba-1⁺ cells in hippocampal DG region.i Quantification of the ratio of CD68 and CD206 in hippocampal DG region. Values are mean ± SD, n = 9 slices from 3 animals per group, *P < 0.05 vs sham group, Student’s t test