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. 2020 Aug 20;17:244. doi: 10.1186/s12974-020-01919-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Knockdown of miR-195 prime microglial/macrophage polarization to M1 phenotype in rats. a MiR-195 expression was detected by qRT-PCR in the hippocampus of rats following the stereotaxic injection of lenti-pre-AMO-miR-195 and/or lenti-pre-miR-195 into CA1 region. Bars represent the mean ± SD. n = 6. *P < 0.05 vs NC group, ^#P < 0.05 vs AMO-195. b Changes of miR-195 affect the percentage of CD68 in the Iba-1⁺cells in hippocampal DG, CA1 region of rats. c Changes of miR-195 has no effects on the percentage of CD206 in the Iba-1⁺cells in hippocampal DG, CA1 region of rats. d Changes of miR-195 regulate the ratio of CD68/CD206 in the hippocampal DG and CA1 regions. Bars represent the mean ± SD. n = 9 slices from 3 animals per group, *P < 0.05 vs NC group, ^#P < 0.05 vs AMO-195. e, f Representative images of CD68 and CD206 expression in the Iba-1⁺ cells of rat hippocampal DG (e) and CA1 (f) region following the stereotaxic injection of lenti-pre-AMO-miR-195 and/or lenti-pre-miR-195 into CA1 region by immunofluorescence staining. The scale bar was 40 μm. g The mRNA expression of TNF-α in the hippocampus of rats following the stereotaxic injection of lenti-pre-AMO-miR-195 and/or lenti-pre-miR-195 into CA1 region. h The mRNA expression of IL-1β in the hippocampus of rats following the stereotaxic injection of lenti-pre-AMO-miR-195 and/or lenti-pre-miR-195 into CA1 region. i The mRNA expression of TGF-β in the hippocampus of rats following the stereotaxic injection of lenti-pre-AMO-miR-195 and/or lenti-pre-miR-195 into CA1 region. Bars represent the mean ± SD. n = 6. *P < 0.05 vs NC group, ^#P < 0.05 vs AMO-195. All data were analyzed using one-way ANOVA followed by Tukey test