Figure 11.
Cholesterol or oleic acid addition experiment. Gain-of-function study was conducted by the addition of cholesterol+ (55 mg), cholesterol++ (110 mg), oleic acid + (45 mg), and oleic acid ++ (200 mg) compared to the negative control (solvent only). Cholesterol or oleic acid were added after preincubation, day 2, and chick embryo was analyzed postinjection, day 4. Vessel morphology was further analyzed using bioinformatics tools including Angiogenesis Analyzer. Scale bar = 5 mm.
