Figure 8.
GCMS analysis of the vessel area at 25 min. (A) Molecules in the white circular area at 25 min were analyzed by their molecular weight and fragmentation pattern. The fragmentation pattern of the major peaks is assigned as follows: C4′ = 55, C3 = 72, C4 = 87, C7 = 94, C7′ = 97, C7 = 135, C11 = 150, M+–CO2 = 236, and molecular ion peak M+ = 280. (B) Database search identified the unknown molecule as linoleic acid, shown as a positive control. m/z values of all fragments were compared to an authentic sample of linoleic acid and the known spectrum of linoleic acid from the database.