Figure 1.

Gating strategy to define cell populations. The lymphocytes were gated according to forward and side scatter dot plot (P1). Dead cells were excluded by staining with Live/Dead fixable viability stain 510 (P2). Single cells were gated according to forward height and side scatter forward area (P3). NK cells were defined from the CD3^- gate (P4) on the basis of the expression of CD16 and CD56. NK cells were divided into 3 subsets: CD56^briCD16^−/+ (P5 and P6), CD56^dimCD16⁺ (P7) and CD56^negCD16⁺ (P8). NK cells and subsets were analyzed for surface expression of NKG2A and NKG2C (P9 and P10, respectively). Co-expression of NKG2A and NKG2C NK cells were indicated in P11 and P12 regions. NK = natural killer.