Table 1.

HLA binding affinity of similar 9-amino acids sequences between SARS-CoV-2 and Mycobacterium bovis predicted by a IEDB analysis resource T cell epitope prediction tool.

						Predicted HLAbinding affinity			Predicted HLA binding affinity
Amino acid sequence	SARS-CoV-2	BCG	Gene name inSARS-CoV-2	Protein name in BCG	HLA allele	SARS-CoV-2	BCG	HLA allele	SARS-CoV-2	BCG
Sequence 1	VLGSLAATV	VLGGLAATV	ORF1ab (nsp9)	UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase	HLA-A*02:01	1.1	1.4	HLA-B*52:01	2.2	1.9
Sequence 2	LQGPPGTGK	LLGPPGTGK	ORF1ab (nsp13)	Type VII secretion AAA-ATPase EccA	HLA-A*11:01	4.2	3.6	HLA-A*33:03	5.4	3.3
Sequence 3	QPTSEAVEA	RPTSEAVEA	ORF1ab (nsp2)	PPOX class F420-dependent enzyme	HLA-B*07:02	8.9	2.2	Not predicted
Sequence 4	LAPLLSAGI	LAPLLCAGI	ORF1ab (nsp3)	Alcohol dehydrogenase	HLA-B*52:01	1.1	4.8	HLA-C*01:02	1.3	5.1
Sequence 5	LAPLLSAGI	LAPLLSAGA	ORF1ab (nsp3)	Metal-transporting ATPase	HLA-C*03:04	1.4	3.9	Not predicted
Sequence 6	LVPFWITIA	LGPFWITIA	ORF1ab (nsp4)	Sugar ABC transporter permease	HLA-C*03:04	5.5	8	Not predicted

Different amino acid residues between SARS-CoV-2 and Mycobacterium bovis are underlined. Common HLA-A (A*01:01, A*02:01, A*03:01, A*11:01, A*24:02, A*33:03), HLA-B (B*07:02, B*08:01, B*44:03, B*52:01) and HLA-C (C*01:02, C*03:04, C*05:01, C*06:02, C*07:01) were tested for peptide binding affinity. The amino acid sequences of both SARS-CoV-2 and Mycobacterium bovis were analyzed by a computer algorithm (IEDB analysis resource, T cell epitope prediction tool, http://tools.iedb.org/main/tcell/). Only the alleles which showed moderate to high binding affinity (Percentile Rank < 10) for both closely similar peptides are shown in this Table 1. Not predicted indicates that these is no HLA allele which can bind to peptide sequences among tested HLA alleles. Frequency of HLA alleles among humans were searched by a publicly available database (Allele Frequency Net Database, http://www.allelefrequencies.net/hla.asp).