Figure S1.

Bait and Prey Levels in Conditioned Media, Related to Figure 1 and Data S3

(A) Qualitative levels of bait (left) and prey (right) proteins in conditioned media used for screen as assessed by western blot against C-terminal 6xHis and 9xHis tags, respectively. Levels were estimated by comparing band intensity of proteins to a purified ECD-Fc standard on each gel. Un; undetectable.

(B) Qualitative prey levels in conditioned media (panel A) versus quantitative relative AP activity (ng/μl), as determined by AP enzymatic assay. Black lines denote mean.

(C) Relative AP activity (ng/μl), as determined by AP enzymatic assay, for all prey used in pooling experiments. Prey levels span the entire spectrum, from the max AP levels obtained in conditioned media (Rst: 659 ng/μl) using our expression system down to levels below quantitation (bql).