Figure S6.

SPR Validation of LAR-PTPR, IL1AP, and PD-L2 PPIs, Related to Figure 4 and Table 1

(A) SPR sensorgrams for PTPRF-Fc and PTPRD-Fc analytes (2-fold dilutions; 2-2048 nM) binding to SALM1/2/3/4/5 ECD-Fc.

(B) SPR sensorgrams for PTPRF-Fc and PTPRS-Fc analytes (2-fold dilutions; 2-2048 nM) binding to IL1RAP-Fc, IL1RAPL1-Fc and IL1RAPL2-Fc ligands.

(C) SPR sensorgrams for PD-L1-Fc analyte (2-fold dilutions; 16-16000 nM) binding to PD-L1-Fc, PD-L2-Fc and PD-1-Fc ligands. TIE1-Fc ligand, negative control.

RU; resonance units.