Table 1.

SPR-Validated apECIA Screen and PHA Predicted PPIs

Ligand-Fc	Analyte-Fc	apECIA	PHA
TIE1	TrkA	+	N/A
hISLR2	hLEPa	+	N/A
	mLepa	N/A	+
mIslr2	hLEPa	N/A	+
	mLepa	N/A	+
SALM1	PTPRD	N/A	+
	PTPRF	N/A	+
	PTPRS	N/A	+
SALM2	PTPRD	known	N/A
	PTPRF	+	N/A
	PTPRS	+	N/A
SALM3	PTPRD	known	N/A
	PTPRF	+	N/A
	PTPRS	known	N/A
SALM4	PTPRD	N/A	+
	PTPRS	N/A	+
SALM5	PTPRD	known	N/A
	PTPRF	known	N/A
	PTPRS	known	N/A
IL1RAPL1	PTPRS	+	N/A
IL1RAPL2	FGFR3	+	N/A
	PTPRS	+	N/A
DSCAM-CT	PTPRM	+	N/A
	PTPRT	+	N/A
DSCAML1-CT	PTPRM	+	N/A
	PTPRT	+	N/A
KIR2DL5	PVR	known	N/A
IL-6Rα	ISLR2	+	N/A
PSG7	DCC	+	N/A
	DSCAM	+	N/A
PSG9	DSCAM	+	N/A
ICAM5	DCC	N/A	+
	IL-6Rα	N/A	+
	ISLR2	N/A	+
	NEO1	N/A	+
	PRTG	+	N/A
	PUNC	N/A	+
	PUNC e11	+	N/A
WFIKKN2	DCC	N/A	+
	IL-6Rα	N/A	+
	ISLR2	N/A	+
	PRTG	+	N/A
	PUNC	+	N/A
	PUNC e11	+	N/A
PD-L1	PD-L1	N/A	+
PD-L2	PD-L1	known	N/A
LTF	DCC	N/A	+
	IL-6Rα	N/A	+
	ISLR2	N/A	+
	NEO1	N/A	+
	PRTG	N/A	+
	PUNC	N/A	+
	PUNC e11	+	N/A
IGFBPL1	DCC	+	N/A
	IL-6Rα	N/A	+
	ISLR2	N/A	+
	NEO1	N/A	+
	PRTG	N/A	+
	PUNC	N/A	+
	PUNC e11	N/A	+
IGFBP7	DCC	N/A	+
	IL-6Rα	N/A	+
	ISLR2	N/A	+
	NEO1	N/A	+
	PRTG	N/A	+
	PUNC	N/A	+
	PUNC e11	N/A	+

ECD-Fc ligand and ECD-Fc analyte PPIs observed by SPR. apECIA denotes PPI was observed in screen. PHA denotes PPI was predicted by phylogenetic homology analysis. SPR conditions are included in Data S5. See also Figure S6.

^ahLEP and mLEP, monomer protein.