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. 2020 Aug 20;15(8):e0237101. doi: 10.1371/journal.pone.0237101

Fig 4. Quantification of grip strength and muscle fiber histology.

Fig 4

(A) Middle aged (9–10 months) but not mature adult (3–7 months) Scn11a+/L799P mice showed a significant decrease in grip strength compared to age-matched Scn11a+/+ littermates (n = 8–11 per group). (B) Both genotypes did not differ in their body weights (n = 8–11 per group). (C) Both genotypes also did not differ in the composition of slow and fast type muscle fibers of the Musculus tibialis cranialis (n = 6 per group; all Scn11a+/+ mice and 4 out of 6 Scn11a+/L799P used for muscle histology were also used for grip strength testing). (D, E) Representative images of muscle fiber staining using anti-slow skeletal myosin heavy chain antibody of cross sections of the forelimbs of Scn11a+/L799P mice (D) and Scn11a+/+ mice (E) (bar 200 μm) showing positively labeled slow (type 1) myosin isoform muscle fibers (marked by arrows). (F, G) Muscle fiber staining using anti-slow skeletal myosin heavy chain antibody of cross sections of the Musculus tibialis cranialis of Scn11a+/L799P mice (F) and Scn11a+/+ mice (G) (bar 200 μm). Values are mean ± SEM. *p < 0.05.