FIG 4.

FTE cells respond to C. trachomatis infection by increasing secretion of cytokines and expression of surface proteins. Primary human polarized FTE cells were infected with CTE3024-mCherry (MOI = 10). (A) Luminex assay of apical secretions from mock-infected and infected cultures 48 h postinfection. Individual dots represent each donor (N = 3). Statistical analysis performed using a paired t test. *, P < 0.05. (B) Mean fluorescent intensities (MFI) of surface protein expression in mock-infected and infected cultures measured by flow cytometry 48 h postinfection. The infected cultures were then gated for mCherry-negative (Ct⁻) and mCherry-positive (Ct⁺) cells. N = 3 donors with 2 replicates per donor. Statistical analysis was performed using Tukey’s multiple-comparison test. *, P < 0.05; **, P < 0.01; ***, P < 0.005.