C. rodentium possesses a functional eut operon. (A) Schematic of the eut locus. (B) Growth curve of WT, ΔeutR, and complemented C. rodentium strains grown in minimal medium with EA (n = 3). (C) RT-qPCR of eutS expression in WT and ΔeutR
C. rodentium strains grown in the absence or presence of EA (n = 3). (D) RT-qPCR of eutS expression in WT, ΔeutR, and complemented C. rodentium strains grown in the presence of EA (n = 3). (E) qPCR showing enrichment of eutS from in vivo ChIP of EutR::MBP compared to MBP (n = 2). Error bars represent standard deviations (SD). **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. ns, not significant.