FIG 2.

EA sensing and EutR-dependent LEE expression in C. rodentium. (A) Schematic of the LEE pathogenicity island. (B) RT-qPCR of espA expression in WT and ΔeutR C. rodentium in the absence or presence of EA (n = 3). (C) RT-qPCR of ler and espA expression in WT, ΔeutR, and complemented C. rodentium strains grown in the presence of EA (n = 3). (D) EMSA of ler, regA, and bla with EutR::MBP. (E) Competition EMSA using biotin-labeled and/or unlabeled ler probes. Ratios compare amount of labeled probe to amount of unlabeled probe. (F) qPCR showing enrichment of ler and rpoA from in vivo ChIP of EutR::MBP compared to MBP (n = 2). Error bars represent SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001.