FIG 1.

Prewashing cells before EAEC infection increases adherence. Adherence to unwashed colonoids or washed colonoids after 3 h (A) or 6 h (B) of incubation. One-way ANOVA was performed on log-transformed data followed by Bonferroni’s test for multiple comparisons: ***, P ≤ 0.001; ****, P ≤ 0.0001 compared to WT infection after washing. ****, P ≤ 0.0001 for comparisons of each bacterial strain in unwashed versus washed colonoids at each time point, except for the aafA strain at 3 h (not significant). No statistically significant differences were observed between strains using unwashed colonoid monolayers.