Role of AAF/II in adherence to human colonoids is not donor specific. Adherence to colonoid monolayers seeded in collagen-coated wells in a 96-well plate after 3 h of incubation. Lines (70C, 75C, and 80C) derived from three different donors are shown in blue, green, and purple, respectively. One-way ANOVA was performed on log-transformed data followed by Bonferroni’s test for multiple comparisons: *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001, as compared to WT infection of each colonoid line.