FIG 4.

AAF/II mediates adherence to duodenal enteroids. Adherence to duodenal enteroid line DP30 after a 3 h of incubation. (A) Adherence to enteroid monolayers seeded into a collagen-coated wells in a 96-well plate after 3 h of incubation. (B) qPCR for aaiC for quantification of adherent bacteria after 3 h of incubation with colonoids from the 75C line. Six replicates were tested in two independent experiments. One-way ANOVA was performed on log-transformed data followed by Bonferroni’s test for multiple comparisons: *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001, compared to WT.