FIG 6.

Quantification of EAEC adherence with different fixative methods that affect retention of MUC2. The relative number of bacteria in every image was measured with ImageJ using the particle enumeration algorithm. The numbers of particles (representing a bacterial cell) per image were summed to generate the total number of bacteria per field. The effect of the two different fixative treatments on each strain was evaluated using an unpaired t test on log-transformed data: *, P ≤ 0.05; **, P ≤ 0.01.