FIG 8.

Infection with AAF/II-positive EAEC strains decreases TEER. EAEC strains were added to prewashed colonoid monolayers (line 70C) seeded on Transwell supports and incubated for 6 h. Monolayers were then washed with PBS and medium containing antibiotics was added to both apical and basolateral chambers. Cells recovered for 18 h and were then assessed for TEER. A Kruskal-Wallis test was performed followed by a Dunn’s test for multiple comparisons: *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001, compared to WT strain 042.