FIG 2.

(A) Mean percentages of live or dead cells of various Staphylococcus strains displaying binding to PE-conjugated mouse monoclonal anti-human CD44 antibody after exposure to 95°C for 10 min as measured using FCM (n = 2; bars show standard errors of the mean; χ² = 50.15061, df = 4, P < 0.00001). (C) Percentages of cells stained with PI and calcein violet AM (CV) falling into one of four regions (double negative, calcein violet positive only, double positive, and PI positive only) when analyzed using FCM after storage overnight in water or PBS (n = 1; for strains stored in water, χ² = 85.37121, df = 12, P < 0.00001; for strains stored in PBS, χ² = 178.30698, df = 12, P < 0.00001). (B and D) Flow cytographs of calcein violet versus PI fluorescence of S. lentus stored in PBS (B) and water (D).