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. 2020 Aug 18;86(17):e01435-20. doi: 10.1128/AEM.01435-20

FIG 8.

FIG 8

(A) Overlays of the Alexa Fluor 647 Plus fluorescence of E. faecium and S. epidermidis stained with rabbit anti-Enterococcus primary antibody, followed by goat anti-rabbit Alexa Fluor 647 Plus and S. epidermidis preincubated with 40 μl per 100 or FcR blocking reagent prior to staining. (B) E. faecium and S. hyicus stained with primary and secondary antibodies or preincubated with FcR blocking reagent prior to staining. (C to E) Overlays of histograms for the Alexa Fluor 647 fluorescence of various strains stained with Genway (C), EastCoast Bio (D), or ViroStat (E) primary anti-Bacillus endospore commercial antibodies combined with Alexa Fluor 647 Plus-conjugated anti-rabbit secondary antibody or Alexa Fluor 647 Plus-conjugated anti-rabbit secondary antibody alone. Color codes for panels C to E: black, B. cereus; red, S. hyicus; blue, S. aureus; purple, S. epidermidis; green, B. cereus preincubated with FcR blocking reagent; yellow, S. hyicus preincubated with FcR blocking reagent; brown, S. aureus preincubated with FcR blocking reagent; aquamarine, S. epidermidis preincubated with FcR blocking reagent.