TABLE 1.

List of primers used in this study

Primer name	Sequence	Characteristic
Up09715_fw_BamHI	CCTAGGATCCTATCGAAATACTGAAAGTACAGGAG	PluDJC_09715 PluDJC_09720 deletion double mutant
Up09715_rv_ovl	CGTCAGTAGATCTTAAACATGTTTTCCCTTTTTACAATAG	PluDJC_09715 PluDJC_09720 deletion double mutant
Down09720_fw_ovl	GATCTACTGACGTCAGACTCACTGAGGCCAGATG	PluDJC_09715 PluDJC_09720 deletion double mutant
Down09720_rv_EagI	CGTTCGGCCGCATCCAGTCGATAAACCCCTTTG	PluDJC_09715 PluDJC_09720 deletion double mutant
UpfliC_fw_BamHI	ACGGGATCCGGCAACGAATGCATCATG	fliC deletion mutant
UpfliC_rv_ovl	CCCTAGCTGAGCGATTAACGTGCCATAGTTAGAGTTCC	fliC deletion mutant
DownfliC_fw_ovl	GGAACTCTAACTATGGCACGTTAATCGCTCAGCTAGGG	fliC deletion mutant
DownfliC_rv_EagI	ACTCGGCCGCAATCACGGCTCCTTAAC	fliC deletion mutant
Up11885_fw_BamHI	GAGGGATCCCCATATATAACCTCTCCTGA	PluDJC_11885 deletion mutant
Up11885_rv_ovl	CCTGAGCTTGACATAAATCACCTCGACTAG	PluDJC_11885 deletion mutant
Down11885_fw_ovl	AAGCTCAGGCATAATTAATTAAGCCAAGCCAC	PluDJC_11885 deletion mutant
Down11885_rv_EagI	TGACGGCCGGTTGGAATTTCACTGCGCAG	PluDJC_11885 deletion mutant
rpoDqPCR_fwDJC	CGGAAGATATCGTCGATTCCGA	Housekeeping, PluDJC_19710
rpoDqPCR_rvDJC	TGTCGTTAGCGGTTTCTGCT	Housekeeping, PluDJC_19710
chitinqPCR_fwDJC	GGTCGCAATATGACGGTCG	Chitinase for qPCR, PluDJC_11885
chitinqPCR_revDJC	GGCAAATAATGGCGCTTGCT	Chitinase for qPCR, PluDJC_11885
vgrGqPCR_fwDJC	ACAGCTTTATCGCCTGACGTT	vgrG for qPCR, PluDJC_04230
vgrGqPCR_rvDJC	GTCCGTTCGGTGATGCCATT	vgrG for qPCR, PluDJC_04230
flgEqPCR_fwDJC	AGGTGGGACTGGGGGTAAAA	flgE for qPCR, PluDJC_09965
flgEqPCR_rvDJC	ACCGCCTTGCATACGGAAAA	flgE for qPCR, PluDJC_09965
bssSregqPCR_fw	TTTGCAATGTCAGTTGTCAACCA	bssS for qPCR, PluDJC_09560
bssSregqPCR_rv	AACGCATCCTGTTGTAGGCT	bssS for qPCR, PluDJC_09560
fliZqPCR_fw	TTGTCACAAAGCTCTTGACCGT	fliZ for qPCR, PluDJC_10165
fliZqPCR_rv	TGCAAAAACGACATAACGCGA	fliZ for qPCR, PluDJC_10165