FIG 6.

Experimental validation of model predictions in 48-well plate format and interactions of components of the defined media described by the least-squares model. (A) Model predicted values plotted against experimental data for the least-squares model (r² = 0.87). (B) New experimental data for 1-ml culture volumes overlaid against the original least-squares model (r² for new data only = 0.85). Open circles show the training data set; closed red circles show new data. Three biological replicates were assessed for each prediction. (C) Amino acid and trace element interactions. (D) Fructose and amino acid interactions. (E) Disodium phosphate and fructose interactions. (F) Calcium chloride and fructose interactions. (G) Ammonium chloride and potassium sulfate. (H) Ammonium chloride and monosodium phosphate. Each panel represents a two-way interaction. The red (pink) zone is an area where the OD₆₀₀ at 72 h failed to reach 1.9, and the white zone is where it surpassed 1.9. Each contour grid line represents an OD₆₀₀ increment of 0.1. All other medium components were kept at concentrations permitting high growth.