KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| goat anti DCX | Santa Cruz | C18 |
| rabbit anti zif268 | Cell Signaling | 4153; RRID:AB_2097038 |
| anti-Prox1 Alexa Flour 647 | NovusBio | NBP-1-30045AF647 |
| anti-cFOS Alexa Flour 488 | NovusBio | NBP2-50037AF488 |
| Bacterial and Virus Strains | ||
| AAV1.Syn.GCaMP6s | Penn Vector Core | 100843-AAV1; RRID:Addgene_100843 |
| AAV-DJ-Syn-Dnmt3a-T2a-GFP | Stanford Vector Core | # 3097 |
| AAV-DJ-Syn-GFP | Standford Vector Core | # 3028 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Tamoxifen | Sigma-Aldrich | #T5648 |
| Isoflurane | Henry Schein | NDC 11695-6776-2 |
| Critical Commercial Assays | ||
| QIAamp DNA Micro Kit | QIAGEN | 56304 |
| TrueMethyl oxBS kit | Tecan | 0414-32 |
| EpiTect Fast Bisulfite Kit | QIAGEN | 59824 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6 | Jackson Laboratory | Stock No:000664 |
| Mouse: C57BL/6 | Taconic BioSciences | C57BL/6NTac |
| Mouse; Dnmt3af/f | Riken BioResource Center | Kaneda et al., 2004 |
| Mouse: Nestin-cre-ERT2 | Luis Parada of University of Texas Southwestern Medical Center, Dallas, TX | Chen et al., 2009 |
| Oligonucleotides | ||
| Nestin-cre Forward Primer: CAGCCTGTTACGTATAGCCG | Chen et al., 2009 | N/A |
| Nestin-cre Reverse Primer: GAGTCATCCTTAGCGCCGTA | Chen et al., 2009 | N/A |
| 16700 Primer: CCTAAGCACCAGGGTGTGAT | Chen et al., 2009 | N/A |
| 16701 Primer: TCACGGTTGGCCTTAGGGTT | Chen et al., 2009 | N/A |
| Dnmt3af/f Forward Primer CTGGTGATTGGAGGCAGTCCATGCA | Kaneda et al., 2004 | N/A |
| Dnmt3af/f Reverse Primer TAGCTGAGGCTGTCTGCATCGGACA | Kaneda et al., 2004 | N/A |
| Software and Algorithms | ||
| StereoInvestigator | MBF Bioscience | https://www.mbfbioscience.com/stereo-investigator |
| pClamp 10.3 | Molecular Devices | https://moleculardevices.app.box.com/s/rraipn0bep04avbr2tcufxww6at4ort5 |
| FlowJo | Tree Star | https://www.fiowjo.com/solutions/fiowjo/downloads |
| Bismark (v17) | Krueger and Andrews, 2011 | https://www.bioinformatics.babraham.ac.uk/projects/bismark/ |
| R | The R Foundation | https://www.r-project.org/ |
| Fiji | Schindelin et al., 2012 | https://fiji.sc |
| CASAVA 1.8.2 | Illumina | https://www.illumina.com/ |
| Other | ||
| RRBS sequencing data | This paper | IGV custom tracks/ uploaded to GEO: GSE72700 and GEO: GSE150551 |
| RRoxBS sequencing data | This paper | IGV custom tracks/ uploaded to GEO: GSE150551 |
| Single nuclei RNA-seq | This paper | uploaded to GEO: GSE150551 |
| Single nuclei RRBS data | This paper | IGV custom tracks/ uploaded to GEO: GSE150551 |