Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | Vax1flox | PMID:27013679 | RRID:MGI:5796178 | |
| Strain, strain background (Mus musculus) | Gt(ROSA)26Sortm14(CAG-tdTomato)Hze (Ai14) | Jackson Laboratories | RRID:IMSR_JAX:007914 | |
| Strain, strain background (Mus musculus) | CD1 | Charles River | Crl : CD1(ICR) RRID:IMSR_CRL:022 |
|
| Sequence-based reagent | Mouse Vax1 cDNA | GenBank | BC111818 | |
| Sequence-based reagent | Cre mRNA | Miltenyi Biotec | 130-101-113 | |
| Recombinant DNA reagent | pCX-EGFP (plasmid) |
PMID:17934458 | Dr Xavier Morin (CNRS, Aix-Marseille University) | |
| Recombinant DNA reagent | pCX-CRE (plasmid) |
PMID:17934458 | Dr Xavier Morin (CNRS, Aix-Marseille University) | |
| Antibody | Anti-digoxigenin (Sheep polyclonal) | Roche | 11093274910 RRID:AB_514497 |
IHC (1:1000) |
| Antibody | Anti-Pax6 (Rabbit polyclonal) |
Millipore | AB2237 RRID:AB_1587367 |
IF, IHC (1: 1000) |
| Antibody | Anti-Ascl1 (mouse monoclonal) |
BD Biosciences | 556604 RRID:AB_396479 |
IHC (1:100) |
| Antibody | Anti-Ki67 (mouse monoclonal) |
BD Biosciences | 550609 RRID:AB_393778 |
IHC (1:200) |
| Antibody | Anti-Dlx2 (guinea pig polyclonal) |
Prof. K. Yoshikawa, Osaka University, Osaka, Japan | PMID:16707790 | IHC (1: 2000) |
| Antibody | Anti-Calbindin D-28K (Rabbit polyclonal) |
Millipore | AB1778 RRID:AB_2068336 |
IF (1 :1000) |
| Antibody | Anti-Calbindin D-28K (mouse monoclonal) |
Swant | 300 RRID:AB_10000347 |
IF (1:3000) |
| Antibody | Anti- Calretinin (mouse monoclonal) |
Synaptic systems | 214111 RRID:AB_2619906 |
IF (1:1000) |
| Antibody | Anti-Tyrosine hydroxylase (chicken polyclonal) | Avès Labs | TYH, RRID:AB_10013440 | IF (1:1000) |
| Commercial assay or kit | SYBR GreenER qPCR SuperMix | ThermoFisher Scientific | 11762100 | |
| Commercial assay or kit | miScript SYBR Green PCR Kit | Qiagen | 218073 | |
| Commercial assay or kit | miRCURY LNA miRNA PCR Assay | Qiagen | 339306 | |
| Commercial assay or kit | miRNAeasy kit | Qiagen | 217004 | |
| Software, algorithm | ZEN Blue | Zeiss | RRID:SCR_013672 | |
| Software, algorithm | Axiovision imaging system | Zeiss | RRID:SCR_002677 | |
| Software, algorithm | Fiji | http://fiji.sc | RRID:SCR_002285 | |
| Software, algorithm | ImageJ | https://imagej.nih.gov/ij/ | RRID:SCR_003070 | |
| Software, algorithm | R Commander | https://CRAN.R-project.org/package=Rcmdr | RRID:SCR_001905 | |
| Software, algorithm | FlowJo | https://www.flowjo.com/solutions/flowjo | RRID:SCR_008520 |