Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus. musculus) | C57BL/6(J) | Jackson lab | Stock No: 000664 | |
| Genetic reagent (Mus. musculus) | CD45.1. C57BL/6 |
Jackson lab | Stock No: 002014 | |
| Genetic reagent (Mus. musculus) | vTreg53 TCR-tg | Li et al., 2018 | ||
| Genetic reagent (Mus. musculus) | Foxp3-GFP | Gift of Vijay. Kuchroo, (Brigham and Women’s Hospital) | ||
| Cell line (Homo sapiens) |
Jurkat E6.1 | ATCC | Cat# TIB-152 RRID:CVCL_0367 | |
| Cell line (Homo sapiens) |
Lenti-X 293T | Takara Bio | Cat# 632180 | |
| Cell line (Homo sapiens) |
K-562 | ATCC | Cat# CCL-243, RRID:CVCL_0004 | |
| Antibody | anti-CD45.1 (clone A20) | Biolegend | Cat# 110724 RRID:AB_493733 |
FACS (1:100) |
| Antibody | anti-CD45.2 (clone 104) | Biolegend | Cat# 109824 RRID:AB_830789 |
FACS (1:100) |
| Antibody | anti-CD3 (clone 17A2) | Biolegend | Cat#: 100214 RRID:AB_493645 |
FACS (1:100) |
| Antibody | anti-CD4 (clone GK1.5) | Biolegend | Cat#: 100422 RRID:AB_312707 |
FACS (1:100) |
| Antibody | anti-TCR Vα2 (clone B20.1) | Biolegend | Cat#: 127806 RRID:AB_1134188 |
FACS (1:100) |
| Antibody | anti-CD44 (clone IM7) | Biolegend | Cat#: 103032 AB_2076204 |
FACS (1:100) |
| Antibody | anti-CD62L (clone MEL-14) | Biolegend | Cat#: 104418 RRID:AB_313103 |
FACS (1:100) |
| Antibody | anti-CD45 (clone 30-F11) | Biolegend | Cat#: 103126 RRID:AB_493535 |
FACS (1:100) |
| Antibody | anti-CD11b (clone M1/70) | Biolegend | Cat#: 101228 RRID:AB_893232 |
FACS (1:100) |
| Antibody | anti-CD11c (clone N418) | Biolegend | Cat#: 117318 RRID:AB_493568 |
FACS (1:100) |
| Antibody | anti-F4/80 (clone BM8) | Biolegend | Cat#: 123116 RRID:AB_893481 |
FACS (1:100) |
| Antibody | anti-c-Myc Alexa Fluor 488 | Cell Signaling Technology | Cat# 9402 RRID:AB_2151827 |
FACS (1:100) |
| Antibody | anti-CD69 (clone FN40) | Biolegend | Cat# 104508, RRID:AB_313111 | FACS (1:100) |
| Antibody | anti-CD25 (clone M-A25) | Biolegend | Cat# 356110, RRID:AB_2561977 | FACS (1:100) |
| Recombinant protein | Streptavidin APC | Biolegend | Cat#: 405243 | FACS (40–400 nM) |
| Antibody | anti-TCR Vβ4 (KT4) | BD Biosciences | Cat#: 553366 RRID:AB_394812 |
FACS (1:100) |
| Antibody | anti-Siglec-F (E50-2440) | BD Biosciences | Cat#: 552126 RRID:AB_394341 |
FACS (1:100) |
| Antibody | anti-Foxp3 (clone FJK-16s) | ThermoFisher | Cat#: 17-5773-82 RRID:AB_469457 |
FACS (1:100) |
| Antibody | anti-TNFα (clone XT3.11) | BioXCell | Cat#: BE0058 RRID:AB_1107764 |
In vivo 10 μg/g |
| antibody | isotype control IgG (clone HRPN) | BioXCell | Cat#: BE0088 RRID:AB_1107775 |
In vivo 10 μg/g |
| Commercial assay or kit | Streptavidin microbeads | Miltenyi | Cat#: 130-048-101 | 50 μl/sample |
| Commercial assay or kit | Foxp3/Transcription Factor Staining Buffer Set | ThermoFisher | Cat#: 00-5523-00 | |
| Commercial assay or kit | CellTrace Violet dye | ThermoFisher | Cat#: C34557 | |
| Commercial assay or kit | Ultra Sensitive Mouse Insulin ELISA Kit | Crystal Chem | Cat#: 90080 | |
| Chemical compound, drug | D-(+)-Glucose | Sigma Aldrich | Cat#: G8270-1KG | |
| Chemical compound, drug | Humulin R | Lilly | Cat#: U-100 | |
| Chemical compound, drug | Freund’s Adjuvant, Complete | Sigma | Cat#: F5881 | |
| Chemical compound, drug | Freund’s Adjuvant, Incomplete | Sigma | Cat#: F5506 | |
| Software, algorithm | Multiplot Studio | Genepattern | https://www.genepattern.org/modules/docs/Multiplot/2 | |
| Software, algorithm | GSEA | Broad Institute | https://www.gsea-msigdb.org/gsea/index.jsp | |
| Software, algorithm | V-QUEST | IMGT | http://www.imgt.org/IMGT_vquest/user_guide |