Parameter name	Unit	Log2- transform	Used in clustering	Definition	Comment
RMP	mV		y	Resting membrane potential (RMP) - average baseline of all sweeps (sweep - a single trace of voltage response to injected current step) in a trace.
RMP std	mV		n		Used for outlier detection
Input resistance	MΩ		y	Input resistance - regression coefficient between injected current steps and voltage responses, measured over the last 50 ms of each sweep without any action potentials (AP).
SAG DP	mV		y	SAG depolarization (DP) - ratio between steady-state mean voltage computed over the last 50 ms of the current step in sweep with 100 mV membrane voltage and maximum hyperpolarization in the same sweep.
Rheobase current	pA		y	Rheobase current - amplitude of injected current step on which the first AP appeared.
Tau start	ms		y	Tau start or Membrane time constant - base of an exponential curve fit to the −100 mV sweep.
1_spk:Spike count	n	y	y	Number of spikes. From here on 1_spk (first spike) corresponds to the sweep evoked by rheobase current.
1_spk:First Spike Lat	ms	y	y	First Spike Latency or Delay - time interval between the beginning of the current step and that of the first AP.
1_spk:ADP amplitude	mV		y	Afterdepolarization (ADP) amplitude - difference between the afterhyperpolarization (AHP) peak and the most positive membrane voltage during the fast repolarization phase.	Set to 0 if no ADP was detected.
1_spk:ADP latency	ms	y	y	ADP latency - interval between the AHP and ADP peaks.	Set to 0 if no ADP was detected. Constant value of 0.2 (ms) was added to all estimates before log-transform.
1_spk:AP amplitude	mV		y	AP amplitude - difference between the AP threshold and its positive peak.
1_spk:AP HW	ms		y	AP half-width (HW) - time interval between voltage points corresponding to half of AP amplitude during depolarization and repolarization phase.
1_spk:Thresh amplitude	mV		y	Threshold amplitude - voltage point before the first spike after which voltage grew faster than 10 mV/ms.
1_spk:AHP amplitude	mV		y	AHP amplitude - difference between the AP threshold and the most negative membrane potential reached during afterhyperpolarization.
1_spk:AP decay time	ms	y	y	AP decay time - Time from the AP peak to the AHP peak.
Sat:Spike count	n	y	y	Number of spikes. From here on sat (saturation) corresponds to the sweep with the highest number of APs.
Sat:Current Step	pA		y	Saturating Current Step - injected current which evokes maximum number of APs.
Sat:First Spike Lat	ms	y	y	Time interval between the beginning of the current step and that of the first AP.
Sat:Fmax Init	Hz	y	y	Initial maximum frequency - Inverse of the shortest inter-spike interval (ISI) among the first three spikes.
Sat:Fmax SS	Hz	y	y	Steady-state maximum frequency - Inverse of the average mean of the last three ISIs.
Sat:Adaptation ratio	%		y	Fmax SS/Fmax init
Sat:AP amplitude	mV		y	Mean difference between the AP threshold and its positive peak for all spikes.
Sat:AP HW	ms		y	Average time interval between voltage points corresponding to half of AP amplitude during depolarization and repolarization phase.
Sat:Thresh amplitude	mV		y	Mean voltage point after which voltage grew faster than10 mV/ms for all spikes.
Sat:AHP amplitude	mV		y	Mean difference between the AP threshold and the most negative membrane potential reached during afterhyperpolarization.
Sat:AP decay time	ms	y	y	Mean time from the AP peak to the AHP peak.