Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Genetic reagent (M. musculus) | Ssttm2.1(cre)Zjh//J; Sst-IRES-Cre | The Jackson Laboratory | RRID:IMSR_JAX:013044 | |
Genetic reagent (M. musculus) | B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J; Ai14 | The Jackson Laboratory | RRID:IMSR_JAX:007914 | |
Genetic reagent (M. musculus) | B6;129S-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J | The Jackson Laboratory | RRID:IMSR_JAX:012569 | |
Strain, strain background (M. musculus) | C57BL/6J | The Jackson Laboratory | 000664; RRID:IMSR_JAX:000664 | |
Antibody | Anti-tyrosine hydroxylase (Rabbit polyclonal) |
Sigma-Aldrich | Cat# AB152, RRID:AB_390204 | IHC: 1:100 |
Antibody | Anti-rabbit IgG H and L Alexa Fluor 488 (Goat polyclonal) | Invitrogen | Cat# A-11008, RRID:AB_143165 | IHC: 1:1000 |
Peptide, recombinant protein | Streptavidin, Alexa Fluor 633 conjugate | Thermo Fisher | Cat# S-21375, RRID:AB_2313500 | (1:1000) |
Software, algorithm | Zeiss ZEN 2 (Blue) | Zeiss | RRID:SCR_013672 | |
Software, algorithm | Leica Application Suite X | Leica | RRID:SCR_013673 | |
Software, algorithm | Fiji Image J | Fiji https://imagej.net/Fiji/Downloads | RRID:SCR_003070; | Version 1.51; Plugins: Neurite Tracer |
Software, algorithm | MATLAB | Mathworks (https://www.mathworks.com/) | RRID:SCR_001622 | Version R2018b; custom script for electrophysiological analysis https://github.com/zubara/fffpa |
Software, algorithm | pClamp | Molecular devices | RRID:SCR_011323 | Clampex 8.2 and 10, Clampfit 10.7 |
Software, algorithm | R Project for Statistical Computing | https://www.r-project.org | RRID:SCR_001905 | Version 3.5; Seurat 2.3.4 https://satijalab.org/seurat/install.html and EWCE (https://github.com/NathanSkene/EWCE) packages for scRNAseq analysis |
Software, algorithm | Python Programming Language | https://www.python.org | RRID:SCR_008394 | Version 3.6; custom script for electrophysiological clustering analysis https://version.aalto.fi/gitlab/zubarei1/clustering-for-nagaeva-et.-al.-sst-vta |
Software, algorithm | CSC Chipster | CSC – IT center for science LTD. (csc.fi) | https://chipster.csc.fi | HISAT2 and HTSeq implementations |
Software, algorithm | Graphpad Prism | Graphpad | RRID:SCR_002798 | Version 8.1 |
Commercial assay or kit | RNAScope probe Mm-Slc6a3 | Advanced Cell Diagnostics | ACD: 315441 | |
Commercial assay or kit | RNAScope probe Mm-Slc17a6 | Advanced Cell Diagnostics | ACD: 319171-C2 | |
Commercial assay or kit | RNAScope probe Mm-Sst | Advanced Cell Diagnostics | ACD: 404638 | |
Commercial assay or kit | RNAScope probe Mm-Slc32a1 | Advanced Cell Diagnostics | ACD: 319191 |