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. 2020 Aug 4;9:e59328. doi: 10.7554/eLife.59328

Key resources table.

Reagent type
(species) or resource
Designation Source or reference Identifiers Additional information
Genetic reagent (M. musculus) Ssttm2.1(cre)Zjh//J; Sst-IRES-Cre The Jackson Laboratory RRID:IMSR_JAX:013044
Genetic reagent (M. musculus) B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J; Ai14 The Jackson Laboratory RRID:IMSR_JAX:007914
Genetic reagent (M. musculus) B6;129S-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J The Jackson Laboratory RRID:IMSR_JAX:012569
Strain, strain background (M. musculus) C57BL/6J The Jackson Laboratory 000664; RRID:IMSR_JAX:000664
Antibody Anti-tyrosine hydroxylase
(Rabbit polyclonal)
Sigma-Aldrich Cat# AB152, RRID:AB_390204 IHC: 1:100
Antibody Anti-rabbit IgG H and L Alexa Fluor 488 (Goat polyclonal) Invitrogen Cat# A-11008, RRID:AB_143165 IHC: 1:1000
Peptide, recombinant protein Streptavidin, Alexa Fluor 633 conjugate Thermo Fisher Cat# S-21375, RRID:AB_2313500 (1:1000)
Software, algorithm Zeiss ZEN 2 (Blue) Zeiss RRID:SCR_013672
Software, algorithm Leica Application Suite X Leica RRID:SCR_013673
Software, algorithm Fiji Image J Fiji https://imagej.net/Fiji/Downloads RRID:SCR_003070; Version 1.51; Plugins: Neurite Tracer
Software, algorithm MATLAB Mathworks (https://www.mathworks.com/) RRID:SCR_001622 Version R2018b; custom script for electrophysiological analysis https://github.com/zubara/fffpa
Software, algorithm pClamp Molecular devices RRID:SCR_011323 Clampex 8.2 and 10, Clampfit 10.7
Software, algorithm R Project for Statistical Computing https://www.r-project.org RRID:SCR_001905 Version 3.5; Seurat 2.3.4 https://satijalab.org/seurat/install.html and EWCE (https://github.com/NathanSkene/EWCE) packages for scRNAseq analysis
Software, algorithm Python Programming Language https://www.python.org RRID:SCR_008394 Version 3.6; custom script for electrophysiological clustering analysis https://version.aalto.fi/gitlab/zubarei1/clustering-for-nagaeva-et.-al.-sst-vta
Software, algorithm CSC Chipster CSC – IT center for science LTD. (csc.fi) https://chipster.csc.fi HISAT2 and HTSeq implementations
Software, algorithm Graphpad Prism Graphpad RRID:SCR_002798 Version 8.1
Commercial assay or kit RNAScope probe Mm-Slc6a3 Advanced Cell Diagnostics ACD: 315441
Commercial assay or kit RNAScope probe Mm-Slc17a6 Advanced Cell Diagnostics ACD: 319171-C2
Commercial assay or kit RNAScope probe Mm-Sst Advanced Cell Diagnostics ACD: 404638
Commercial assay or kit RNAScope probe Mm-Slc32a1 Advanced Cell Diagnostics ACD: 319191