Figure 3. N-terminal domain and the ATP-binding site.

(A) The N-terminal domain resides at the interface between the transmembrane domain, CBS1 and CBS2. N-terminal domain is colored in magenta, transmembrane domain in blue, CBS1 in cyan and CBS2 in green. (B) Sequence alignment of N-terminal domain of ggCLC-7 with human CLC-1, CLC-2, CLC-Ka, CLC-Kb, CLC-3, CLC-4, CLC-5, CLC-6, CLC-7. Positions of ATP coordinating Glu91 and Ser92 in ggCLC-7 are highlighted in magenta. (C) Two views of the ATP binding site in the cytoplasmic domain of ggCLC-7. Side chains that interact with ATP are shown as sticks. Experimental cryo-EM density is shown as blue mesh contoured at 12 σ threshold. Mg²⁺ ion shown as a pink sphere.

Figure 3—figure supplement 1. Comparison of ATP-binding sites in ggCLC-7 and hsCLC-5.

ATP-binding sites in ggCLC-7 (colored by domain) and hsCLC-5 (2J9L) (grey). Residues are labeled according to ggCLC-7 sequence. Mg²⁺ ion shown as a pink sphere.