Table 1. Cryo-EM data acquisition, reconstruction and model refinement statistics.
| ggCLC-7 | hsCLC-7/OSTM1 | ||||
|---|---|---|---|---|---|
| Consensus | Consensus | TMD Focus | LD Focus | CD Focus | |
| Cryo-EM acquisition and processing | |||||
| EMDB accession # | 22386 | 22389 | |||
| Magnification | 22,500x | 22,500x | 22,500x | 22,500x | 22,500x |
| Voltage (kV) | 300 | 300 | 300 | 300 | 300 |
| Total electron | 61 | 44 | 44 | 44 | 44 |
| Exposure (e-/Å2) | |||||
| Exposure time (s) | 8 | 4 | 4 | 4 | 4 |
| Defocus range (µM) | -1.0 to -2.5 | -1.0 to -2.5 | -1.0 to -2.5 | -1.0 to -2.5 | -1.0 to -2.5 |
| Pixel size (Å) | 1.0723 | 1.064 | 1.064 | 1.064 | 1.064 |
| Symmetry imposed | C2 | C2 | C1 | C1 | C1 |
| Initial particles | 4,020,225 | 15,288,379 | 15,288,379 | 15,288,379 | 15,288,379 |
| Final particles | 87,707 | 327,619 | 655,238 | 655,238 | 655,238 |
| Resolution (masked, Å) | 2.93 | 2.82 | 2.85 | 2.79 | 3.04 |
| Density modified CC (0.5, Å) | 2.92 | 2.81 | 2.80 | 2.70 | 3.30 |
| Model refinement | |||||
| PDB ID | 7JM6 | 7JM7 | |||
| Model resolution (Å) | 2.98/2.40 | 2.92/2.56 | |||
| FSC threshold | 0.50/0.143 | 0.50/0.143 | |||
| Model refinement resolution | 300-2.9 | 300-2.8 | |||
| RMS deviations | |||||
| Bond length (Å) | 0.005 | 0.005 | |||
| Bond angle (°) | 0.806 | 0.769 | |||
| Ramachandran plot | |||||
| Favored (%) | 97.73 | 97.87 | |||
| Allowed (%) | 2.27 | 2.13 | |||
| Disallowed (%) | 0 | 0 | |||
| Rotamer Outliers (%) | 0.00 | 1.17 | |||
| Validation | |||||
| MolProbity score | 1.37 | 1.37 | |||
| Clashscore | 5.68 | 5.38 | |||