Figure 4.

Evaluating HNRNPH expression by tissue microarray. (A) Reactive tonsil tissue stained for HNRNPH expression was used as the reference. Representative images of tumor cores scored as weak (0), moderate (1), or strong (2) HNRNPH staining. (B) Based on the tissues on this tissue microarray with available RNA-seq data (n = 79), higher intensity of HNRNPH staining was associated with a lower splice ratio or a relatively lower proportion of alternative (unproductive transcripts; P = .01376, Wilcoxon rank sum). (C) For the same cases as in panel B, cases with higher intensity staining did not have higher levels of HNRNPH1 mRNA based on normalized read counts. In contrast, cases with high-intensity staining were associated with lower HNRNPH1 expression (P = .02004; Wilcoxon rank sum). (D) Stratification of patients by moderate or strong HNRNPH staining did not show a significant association with OS, but there was a trend toward inferior outcomes.