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. 2020 May 9;26(9):1353–1367. doi: 10.1093/ibd/izaa064

FIGURE 1.

FIGURE 1.

MUC5AC/Muc5ac gene expression increased at the mucosal surface in active UC and in murine colitis. A and B, Colonic mucosal biopsies collected from healthy control patients (normal) or from sites of endoscopically visible inflammation in patients with UC (inflamed UC). Total RNA extracted and Taqman RT-PCR for MUC5AC (A) and MUC2 (B) performed. Transcript levels calculated relative to 18S and expressed as fold change compared to normal. Data displayed as mean ± SEM. Results represent n = 11 to 14 normal samples and n = 10 to 12 inflamed UC. Statistical difference assessed by 2-tailed Mann-Whitney U test. C and D, Administered DSS (3%) or water to C57 Black 6 mice. After 6 days, mucosal scrapings from the distal colon harvested and total RNA extracted. TaqMan RT-PCR for Muc5ac (C), Muc2 (D), and 18S performed. Transcript levels calculated relative to 18S and expressed as fold change compared to water control. Data displayed as mean ± SEM. Results represent n = 5 to 8 mice per group. Statistical differences calculated using 2-tailed unpaired Student t test. SEM indicates standard error of the mean. *P< 0.05, ***P< 0.0001.