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. 2020 May 9;26(9):1353–1367. doi: 10.1093/ibd/izaa064

FIGURE 3.

FIGURE 3.

Loss of Muc5ac results in increased neutrophil frequency and cytokine concentration in the colonic lamina propria during experimental colitis. A and C, Administered DSS (3%) or water to Muc5ac-deficient mice (Muc5ac-/-) and their wildtype control mice (Muc5ac+/+). After sacrifice on day 7, post-DSS whole colon digested for cytokine analysis by Meso Scale. Data displayed relative to protein concentration as determined by BCA. Results represent 1 individual experiment with n = 9 to 13 mice/group. Statistical differences determined by unpaired Student t test. B, Administered DSS (3%) or water to Muc5ac-/- and Muc5ac+/+. Following sacrifice on day 7 or 9, post-DSS whole colon digested for flow cytometric analysis to identify neutrophils (% live CD45.2+ MHCIINeg SiglecFNeg Ly6G+ cells). Results represent 2 independent experiments with n = 3 mice/water group and n = 8 mice/DSS group. Two-way ANOVA with posthoc Bonferroni t test used to determine statistical differences. In all cases, data displayed as mean ± SEM. ANOVA indicates analysis of variance; SEM, standard error of the mean. *P< 0.05.