Skip to main content
. 2020 May 9;26(9):1353–1367. doi: 10.1093/ibd/izaa064

FIGURE 5.

FIGURE 5.

Antibiotic treatment abrogates differences between Muc5ac-deficient (Muc5ac-/-) and wildtype (Muc5ac+/+) mice during experimental colitis. Muc5ac-/- mice and Muc5ac+/+ mice were gavaged daily with antibiotic cocktail (ampicillin 1 mg/mL, gentamicin 1 mg/mL, metronidazole 1 mg/mL, neomycin 1 mg/mL, and vancomycin 0.5 mg/mL) or vehicle for 10 days before (day -10) administration of DSS (3%), with continued daily delivery of antibiotics or vehicle during DSS. A, Weight measurements obtained for each group of mice and displayed as percentage of body weight at day 0 of antibiotic administration. B, Following sacrifice on day 7, post-DSS colons harvested and measured. C, Representative histological sections from colon harvested at day 7 post-DSS (bar represents 200 µm; images acquired at 10x). D, Histological analysis of whole colon (proximal and distal combined scores) harvested at day 7 post-DSS provided by pathologist blinded to the groups and the study. E and F, Following sacrifice on day 7, post-DSS whole colon digested for cytokine analysis by Meso Scale. Data displayed relative to protein concentration as determined by BCA. Results represent 2 independently performed experiments and displayed as mean ± SEM; n = 7 to 17 mice/group. Two-way ANOVA with posthoc Bonferroni t test used to determine statistical differences. ANOVA indicates analysis of variance; SEM, standard error of the mean. *P< 0.05.