Skip to main content
. 2020 Aug 20;11(8):665. doi: 10.1038/s41419-020-02893-0

Fig. 5. CASC4 overexpression decreases cell migration and invasion.

Fig. 5

a Schematic representation of CASC4-WT and amino acid mutations used for the generation MDA-MB-231 stable cells. b Western blot analysis of lysates from MDA-MB-231 stable cells expressing pIRES-empty vector, CASC4-WT-V5, or CASC4-5REL-V5. c, d Wound healing assay images and quantifications after 6 h, 12 h post-cell monolayer scratching in MDA-MB-231 stable cell lines. e–h The ability of MDA-MB-231 stable cells to migrate (6 h) (e–f) or invade (16 h) (g–h) was assessed by counting the number of cells stained with DAPI on the underside of a polycarbonate membrane under a phase contrast microscope (×20). These results are representative of at least three independent experiments. Error bars indicate averaged values ± standard error from the mean (SEM). P-values: *P ≤ 0.05, ***P ≤ 0.001, n.s. not significant (two-sided Student’s t-test).